ELUTRAP MANUAL PDF
The Elutrap system is designed to isolate nucleic acids and proteins from agarose or polyacrylamide gel slices by electroelution. Samples are purified with . azim58 – Official elutrap documentation. Elutrap protocol. “C:\kurt\storage\CIM Research Folder\DR\\\ELUTRAP\ “. The Elutrap, seen from above, is placed on a horizontal agarose gel electrophoresis chamber. Outer limits of the trap are defined with disposable membranes at.
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Assembly of the Elutrap system is easy. To clean, discard the buffer down the drain. The same applies to handling of the equipment. BT1 Membranes Whatman No.: However, take precautions to keep the BT2 membrane dry for as long as possible. However, the Elutrap electrophoresis chamber allows for the most efficient flow of current through the device and can be used for up to four samples simultaneously. Do not stack gel slices above the device height. Leakage can be detected by the appearance of water on the paper filter.
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Whatman Elutrap Electroelution System, Whatman 10404090 Replacement Components BT1 Membranes
Allows up to four different samples to be run simultaneously. Please add ” labplanet. Gel pieces containing the purified template. Please change the country on your profile in order to switch to another country store. About Me biotechie I am a Master’s student flutrap Biotechnology and this is my attempt to share experiences and lessons learnt from school.
Conclusion Organic solvents may damage the device and should be removed promptly.
Elutrap Electrophoresis Chamber – GE Healthcare Life Sciences
Dialysis membrane — one per each device used. The Elutrap system is designed to isolate nucleic acids and proteins from agarose or polyacrylamide gel slices by electroelution. The collection chamber end of the device should be placed near the open aperture at the end of the channel towards the positively charged electrode.
Place the dialysis membrane between the screw and the closed U insert in the large chamber of the device.
Learn more about our Return Policy. It is essential to then autoclave the buffer to remove all traces of DEPC. Remember to cut the dialysis membrane with one edge slightly higher than the other similar to the shape of the BT1 membrane. Take the BT1 membrane out using forceps and clean thoroughly with DI water to remove all traces of glycerol.
Elutrap Starter Kit with electrophoresis chamber includes one Elutrap device, one electrophoresis chamber, 50 BT1 membranes, and 50 BT2 membranes. If you cannot enable JavaScript or you are having technical difficulties, please contact us — we are always here to help! We offer only genuine Whatman products, authorized for sale in the U. Place the tray against the tray stop so that the bottom aperture on the tray lies over the buffer.
The Elutrap system can be used with other horizontal gel electrophoresis chambers. Elutrap Electrophoresis Chamber Replacement electrophoresis chamber for the Elutrap Electroelution system. Careful quick collection is desirable.
Gel slices should not extend beyond 6 cm in the sample chamber. It is very important to place the sloping surface of the BT1 membrane next to the triangular mark on the side of the device.
The buffer in the electrophoresis chamber should cover the electrodes. We however use a single layer in the device. Always keep the BT1 and the manjal membrane moist.
Web browser based cookies allow us to customize our site for you, save items in your cart, and provide you with a great experience when shopping LabPlanet. Turn off the unit after completion of the elution.