Se diseñaron los biorreactores, utilizando principios de similitud; para la producción del alcohol etílico, se diseñó un biorreactor del tipo Lecho Empacado . Inmovilización de levaduras en residuos lignocelulósicos para la producción de etanol en biorreactor de lecho empacado. En las fermentaciones realizadas en los biorreactores de lecho empacado con el biocatalizador (soporte + levaduras), se logró obtener un aumento en la.

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Analytic techniques are also required for measuring the physicochemical properties of yeast cells and carriers, to determine the mechanisms of immobilization that occur in the process.

The corn leave carrier was the material in which the lowest amount of cells was immobilized. We kindly thank the Biotechnology and Biotransformation Groups of the Universidad de Antioquia for their support. An important result obtained from the experimental design, is about selection of the best material for cell immobilization.

We recommend downloading the newest version of Flash here, but we support all versions 10 and above. Two different types of bioreactors were designed, using principles of similarity.

Used as reactor vessel R. Enzyme Microbiology and Technology. Table 3 shows the values of productivity reached with the other materials. A protocol for packaging materials and a quantification methodology of immobilized biomass was developed.

The potential use of raw materials obtained from agro-industrial waste as supports for cell immobilization was recently evaluated. We also evaluated the glucose syrup obtained from cassava flour as alternative substrate. Carriers for immobilization The lignocellulosic waste used were wood shaving, cane bagasse, corn leave, and corn cob, which were obtained from some agro industries in the region.

Biology of Microbial Communities – Interview. Memorias al Congreso Nacional.


Modelado de reactores de lecho empacado en COMSOL®

Applied and environmental microbiology, 68 7pp. The immobilization matrixes more frequently used have been polymeric gels like alginate, where the cells are immobilized for entrapment into the gel. Study of the effect of size and flow rate for each material To determine the effect on immobilization of flow rate and the carrier size, we performed a factorial experimental design with 2 factors and 2 levels, and a central point with three replicates. In this study we developed a protocol for quantification of immobilized biomass on lignocellulosic carriers and we obtained reproducible results.

The productivities reached in fermentations with cells immobilized on corn leaves, corn cob, and wood shaving are similar to each other.

The treatment corresponding to a flow rate of 1. The average sizes of the pieces obtained were 3. We studied various techniques spectrophotometry, protein quantification and dry weight ; the better results were obtained with the dry weight technique, which was modified to emapcado direct quantification of biomass immobilized on the carrier. Ciudad Universitaria Calle 67 No. The Scanning Electron Microscopy technique SEM was used to observe the differences in the structures of materials and cell immobilization.


We can see that the amount of immobilized cells varies depending on the treatment and the material. Before starting the continuous process, we carried out the stages of cell immobilization and batch fermentation previously described. Preparar el inoculante algas.

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El RT se compone de un vaso de precipitados de vidrio de ml. Based on these results, the best choice of flow rate and size for cell immobilization can be established considering the best operating conditions of the reactor, because the values of the studied ranges for flow rate and size have no effect on the amount of immobilized biomass in the carriers. The choice of the carrier sizes and flow rates for immobilization in fermentation conditions within the studied ranges can be made by considering the best operating conditions.


You must be signed in to post a comment. The size of the carrier selected was 3. We evaluated four different waste lignocellulosic materials: The value obtained was 0.

The fermenting medium used for the experiments was the following: In addition, the results have high deviations. Dentro de la zona de la biomasa, el total de densidad de la biomasa y la biomasa de algas fueron consistentes en el transcurso de este estudio. An unexpected error occurred.

There is a similar behavior in terms of substrate consumption obtained in the fermentations presented in figure 5. Fill out the form below to receive a free trial or learn more about access:.

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Como tal, el rendimiento del reactor en aplicaciones ampliadas es actualmente desconocido. From Whole Cell Immobilization: The production of ethanol obtained at the output of the reactor was These immobilization mechanisms are not only dependent of the surface characteristics of the carriers, they depend on cell surface characteristics, the carrier used and the operational conditions under which the immobilization is carried out flow, temperature, etc.

Figure 1 shows the results obtained in the cell immobilization performed on the 4 carriers evaluated. The experiments were performed on a laboratory level in column reactors of mL. JavaScript is disabled for your browser. The results have established the implementation potential of ethanol production by continuous fermentation with immobilized cells in waste lignocellulosic materials.